
2023年5月26日Peter Qin教授讲座
题目:Unveiling Conformational Checkpoints Regulating CRISPR Target Discriminations
报告人:Peter Qin,美国南加利福尼亚大学化学系 教授
报告时间:2023年5月26日下午15:00
报告地点:化学材料综合楼B407-409
主持人:毛宗万 教授
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报告人简介:
Prof. Peter Qin is the chairman of department of Chemistry at University of Southern California. They are interested in understanding the mechanisms of specific recognition of nucleic acids that inform genome engineering as well as developments in diagnostics and therapeutics. They use Site-Directed Spin Labeling (SDSL) and other tools to study the relationship between structure, dynamics, and function in nucleic acids and protein-nucleic acid complexes.
报告内容简介:
CRISPR (Clustered-Regularly-Interspaced-Short-Palindromic-Repeats) systems have been adapted into programmable agents for genome-wide manipulation of nucleic acids in many organisms, unleashing a revolution in genome editing and manipulation that is still rapidly advancing. Fundamental understanding on mechanisms of CRISPR target discrimination provides the foundation for CRISPR revolution, and is critical for overcoming remaining obstacles, such as the “off-target effects” that result in undersigned aberrant actions. Our group has been studying mechanisms of CRISPR-Cas9 and Cas12a that target double-stranded DNA. In this presentation, I will describe work on identifying conformational checkpoints contributing to DNA target recognitions in Cas12a and Cas9 using site-directed spin-labeling in conjunction with fluorescence spectroscopy and enzymology. The work elucidates connections between DNA physical properties and CRISPR activities that may be explored in CRISPR-based genome manipulation applications.
代表文章:
1. Shi, F., F. Kong, P. Zhao, X. Zhang, M. Chen, S. Chen, Q. Zhang, M. Wang, X. Ye, Z. Wang, Z. Qin, X. Rong, J. Su, P. Wang, Qin, P.Z.,* and Du, J.* (2018), "Single DNA Electron Spin Resonance Spectroscopy in Aqueous Solutions." Nat. Meth., 15, 697-699.
2. Hu, Y.; Wang, T.; Singh, J.; Sun, R.; Xu, L.; Niu, X.; Huang, K.; Bai, G.; Liu, G.; Zuo, X.; Chen, C.; Qin, P.Z.; Fang, X.*; (2022) "Phosphorothioate-Based Posttranscriptional Site-Specific Labeling of Large RNAs for Structural and Dynamic Studies." ACS. Chem. Biol., 17, 2448–2460.
3. Li, Y., Liu, Y., Singh, J., Tangprasertchai, N.S., Trivedi, R., Fang, Y., and Qin, P.Z.* (2022) "Site-Specific Labeling Reveals Cas9 Induces Partial Unwinding Without RNA/DNA Pairing in Sequences Distal to the PAM." CRISPR J., 5, 341-352.
4. Wang, Y., Kathiresan, V., Chen, Y., Hu, Y., Jiang, W., Bai, G., Liu, G., Qin, P.Z.*, Fang, X.*, (2020), "Posttranscriptional site-directed spin labeling of large RNAs with an unnatural base pair system under non-denaturing conditions." Chem. Sci., 11, 9655-9664.
5. Cao, Q., Li, Y., Freisinger, E., Qin, P.Z.*, Sigel,R.K.O.*, and Mao, M.Z.*, (2017) “G-quadruplex DNA targeted metal complexes acting as potential anticancer drugs.” Inorg. Chem. Front., 4, 10-32.