Designed DNA origami nanostructures for imaging, analysis, and material applications
Masayuki Endo教授在DNA纳米结构应用上做出了重要贡献。在此次报告中，他将介绍DNA origami结构在成像，分析和材料中的应用。
DNA nanotechnology and nanoscience are emerging research fields in the past two decades. DNA origami, programmed self-assembly system, allows us the design and construction of various structures with functions in a nanoscale precision. We are employing this technology for single-molecule observation and analysis and construction of nanodevices. We have developed AFM-based single-molecule observation systems for biomolecule imaging by employing DNA origami nanostructures and high-speed AFM (1,2). Using this system, we have visualized and analyze the DNA structural changes and enzyme reactions at the single-molecule resolution. For the analytical applications, biomolecules in the confined environment were investigated using optical tweezers. We created a nano-sized space using DNA origami, and found that DNA molecules placed in the nanospace showed unique properties, in which DNA molecules were thermodynamically stabilized and the folding occurred rapidly (3). In addition, the properties of these confined spaces were characterized (4). By integrating and arranging the molecules on the DNA origami, we created DNA motor system and observed the detailed movement using HS-AFM (5,6). The transcription regulation system was also created by placing RNA polymerase and gene of interest, whose activity could be precisely controlled by distance between these molecules (7). We also created molecular switches and genetic circuit using this system. For control of dynamic 3D structures, we incorporated photoresponsive system to switch their mechanical movements (8). The photoresponsive plasmonic nanodevice was also constructed using the dynamic nanostructure (9). For the material applications, controlled assembly of the DNA origami nanostructures is one of the on-going targets for creation of the large-sized DNA architectures. The study on the assembly processes is still challenging for further creation of micrometer-scale DNA materials. We developed a novel strategy using a lipid bilayer to assemble various DNA origami monomers into 2D lattices with micrometer size (10). The origami structures were mobile on the lipid bilayer surface, and the dynamic processes including attachment and detachment of DNA origami monomers and reorganization of lattices were visualized using high-speed AFM. Furthermore, the square-shaped DNA origami was assembled into the lattices in a programmed fashion and dynamic assembly processes was visualized (11).
The methods we have developed provide strategies for creation of novel designed architectures with various functions and integrated molecular systems.
1. M. Endo, H. Sugiyama, Acc. Chem. Res. 47, 1645-1653 (2014).
2. A. Rajendran, M. Endo, H. Sugiyama, Chem. Rev. 114, 1493-1520 (2014).
3. P. Shrestha, M. Endo, H. Mao, et al., Nature Nanotechnology, 12, 582–588 (2017).
4. S. Jonchhe, M. Endo, H. Mao, et al., Proc. Natl. Acad. Sci. USA 115, 9539-9544 (2018).
5. S. F. J. Wickham, M. Endo, A. J. Turberfield, et al., Nature Nanotechnology, 7, 169-173 (2012).
6. S. F. J. Wickham, M. Endo, A. J. Turberfield, et al., Nature Nanotechnology, 6, 166-169 (2011).
7. T. Masubuchi, M. Endo, H. Tadakuma, et al., Nature Nanotechnology, 13, 933-940 (2018).
8. E. M. Willner, H. Dietz, M. Endo, et al., Angew. Chem. Int. Ed. 56, 15324-15328 (2017).
9. A. Kuzyk, M. Endo, N. Liu, et al., Nature Commun. 7, 10591 (2016).
10. Y. Suzuki, M. Endo, H. Sugiyama, Nature Commun. 6, 8052 (2015).
11. Y. Suzuki, H. Sugiyama, M. Endo, Angew. Chem. Int. Ed. 57, 7061-7065 (2018).