2018年8月28日卡内基梅隆大学助理教授招永欣学术报告

Expansion Microscopy: A New Way to Visualize Invisible Secrets of Biological Systems

发布人:高级管理员 发布日期:2018-08-21
主题
Expansion Microscopy: A New Way to Visualize Invisible Secrets of Biological Systems
活动时间
-
活动地址
丰盛堂芙兰学术中心A403
主讲人
招永欣助理教授
主持人
杨振宇教授

报告主题:Expansion Microscopy: A New Way to Visualize Invisible Secrets of Biological Systems

报告人:招永欣助理教授 卡内基梅隆大学

主持人:杨振宇教授

时间:2018年8月28日 10:30-11:30

地点:丰盛堂芙兰学术中心A403

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报告摘要:

Expansion Microscopy: A New Way to Visualize Invisible Secrets of Biological Systems

Yongxin (Leon) Zhao

Department of Biological Sciences, Carnegie Mellon University

All the biological systems are configurations of molecular building blocks (e.g., proteins, nucleic acids, lipids, and carbohydrates) that are assembled with nanoscale precision. Characterization of such configurations in nanoscale is critical for understanding mechanisms underlying biological functions and diseases. In modern biology, diffraction-limited microscopy is used to examine various biological specimens. However, diffraction-limited microscopy is unable to resolve distinct molecular species and their nanoscale configurations below the diffraction limit, which severely limited its capability of analyzing intricate and subtle biological changes. Recently, Expansion Microscopy (ExM) has emerged as a ground-breaking new principle for scalable, nanoscale optical imaging of biological specimens. ExM works by chemically transforming biological tissues into a tissue-gel hybrid and magnifying them physically, rather than optically. Typical ExM protocols expand tissues by 100 folds in volume, thus enabling nanoscale optical imaging with resolution ~60 nm using diffraction-limited microscopes. I will give an overview of current ExM tools and their applications on specimens from model animals and human biopsies, and then I will briefly introduce some of the next generation ExM tools under development in the Zhao Biophotonics Lab.

2018年8月28日卡内基梅隆大学助理教授招永欣学术报告

 

报告人简介:

Yongxin (Leon) Zhao is an Assistant Professor in the Department of Biological Sciences at Carnegie Mellon University and an alumni of Sun Yat-Sen University. His scientific training began with a B.Sc. in the School of Chemistry at Sun Yat-Sen University. He received his Ph.D. from University of Alberta, where he developed genetically encoded fluorescent indicators for calcium ions (GECO family, Science 2011) and membrane potential (QuasAr family, Nature Methods 2014), under guidance of Drs. Robert E. Campbell and D. Jed Harrison. Dr. Zhao then conducted his postdoctoral research at the Synthetic Neurobiology group of Dr. Edward S. Boyden in the Media Lab at Massachusetts Institute of Technology, where he developed Expansion Pathology, a pathology-optimized tissue expansion technique for nanoscale imaging of clinical specimens (Nature Biotechnology 2017). Since he joined the faculty at Carnegie Mellon University in 2017, Dr. Zhao has established a research program to develop new imaging tools for 3-D molecule mapping in biological systems with nanoscale precision as well as recording of the dynamics of biological processes and deploy them to understand the fundamental mechanisms underlying complex biological systems and diseases and foster new diagnoses and therapeutics.

 

Selected Publicat

1.A. T Wassie (equal contribution), Y. Zhao (equal contribution), E. S Boyden*, ‘Expansion Microscopy: Principles and Uses in Biology and Medicine’, Nature Methods, accepted.

2.Y. Zhao (equal contribution), O. Bucur (equal contribution), et al., ‘Nanoscale imaging of clinical specimens using pathology-optimized expansion microscopy.’ Nature Biotechnology, 2017, 35 (8), 757–764.

3.D. R. Hochbaum (equal contribution), Y. Zhao (equal contribution), et al., ‘All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins’, Nature Methods, 2014, 11, 825–833.

4.P. Zou (equal contribution), Y. Zhao (equal contribution), et al., ‘Bright and fast voltage reporters across the visible spectrum via electrochromic FRET (eFRET)’, Nature Communication, 2014, 5, Article number: 4625.

5.Y. Zhao, S. Araki, et al., “An Expanded Palette of Genetically Encoded Ca2+ Indicators”. Science, 2011, 333, 1888-1891.

 

 

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